pANC79a(P4-GGGSGGG-cTEV-HA-IRES-dTomato)
(Plasmid
#248356)
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PurposeLentiviral vector that can express P4 along with C terminal split TEV. Expressed off of CMV tet ON promoter with dTomato marker
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248356 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepLenti
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameP4-GGGSGGG-cTEV-HA
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SpeciesSynthetic
- Promoter tight TRE
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Dimerizes with P3-nTEV construct, Addgene Plasmid # 248357.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pANC79a(P4-GGGSGGG-cTEV-HA-IRES-dTomato) was a gift from Arnab Mukherjee (Addgene plasmid # 248356 ; http://n2t.net/addgene:248356 ; RRID:Addgene_248356) -
For your References section:
A programmable genetic platform for engineering noninvasive biosensors. Chacko AN, Dhanabalan KM, Wan J, Chien R, Anderson NT, Xu B, Pham K, Tiwari R, Mukherjee A. Sci Adv. 2026 Jan 16;12(3):eaec1211. doi: 10.1126/sciadv.aec1211. Epub 2026 Jan 14. 10.1126/sciadv.aec1211 PubMed 41533785