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pANC98c(3xFLAG-NES-FRB-(G4S)4-cTEV(219)-IRES-EBFP)
(Plasmid #248359)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248359 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLenti
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    3xFLAG-NES-FRB-(G4S)4x-cTEV(219)
  • Species
    Synthetic
  • Promoter tight TRE

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Dimerizes with FKBP-nTEV construct in presence of rapamycin, Addgene Plasmid # 248358. Please visit https://doi.org/10.1101/2025.09.07.674790 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pANC98c(3xFLAG-NES-FRB-(G4S)4-cTEV(219)-IRES-EBFP) was a gift from Arnab Mukherjee (Addgene plasmid # 248359 ; http://n2t.net/addgene:248359 ; RRID:Addgene_248359)

  • For your References section:

    A programmable genetic platform for engineering noninvasive biosensors. Chacko AN, Dhanabalan KM, Wan J, Chien R, Anderson NT, Xu B, Pham K, Tiwari R, Mukherjee A. Sci Adv. 2026 Jan 16;12(3):eaec1211. doi: 10.1126/sciadv.aec1211. Epub 2026 Jan 14. 10.1126/sciadv.aec1211 PubMed 41533785
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