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pCTK055
(Plasmid #248418)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248418 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pYTK001
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RiboJ60
  • Species
    E. coli
  • Mutation
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    A. A. K. Nielsen et al., 'Genetic circuit design automation', Science, vol. 352, no. 6281, p. aac7341, Apr. 2016, doi: 10.1126/science.aac7341

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Type 2c parts are insulating ribozymes in the CTK system

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCTK055 was a gift from Heinz Koeppl (Addgene plasmid # 248418 ; http://n2t.net/addgene:248418 ; RRID:Addgene_248418)

  • For your References section:

    The Coli Toolkit (CTK): An Extension of the Modular Yeast Toolkit for Use in E. coli. Mejlsted J, Kubaczka E, Wirth S, Koeppl H. ACS Synth Biol. 2026 Jan 25. doi: 10.1021/acssynbio.5c00489. 10.1021/acssynbio.5c00489 PubMed 41581077
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