pCTK119
(Plasmid
#248482)
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Purpose(Empty Backbone) Encodes p15A ori-KanR-LacI-TetR [GFP] as a Type 678 part to be used in the CTK/YTK systems
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248482 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonep15A
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Type 678 parts are backbones in the CTK system
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCTK119 was a gift from Heinz Koeppl (Addgene plasmid # 248482 ; http://n2t.net/addgene:248482 ; RRID:Addgene_248482) -
For your References section:
The Coli Toolkit (CTK): An Extension of the Modular Yeast Toolkit for Use in E. coli. Mejlsted J, Kubaczka E, Wirth S, Koeppl H. ACS Synth Biol. 2026 Jan 25. doi: 10.1021/acssynbio.5c00489. 10.1021/acssynbio.5c00489 PubMed 41581077