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pJB127
(Plasmid #248630)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248630 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pJB94
  • Backbone manufacturer
    Joseph Sorg, Addgene plasmid #208752
  • Total vector size (bp) 8768
  • Vector type
    Other

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    sacB
  • Alt name
    levansucrase
  • Insert Size (bp)
    1422
  • Entrez Gene
    sacB (a.k.a. BSU_34450, BSU34450)

Gene/Insert 2

  • Gene/Insert name
    codA
  • Alt name
    cytosine deaminase
  • Species
    E. coli
  • Insert Size (bp)
    1284
  • GenBank ID
    CP184745.1

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJB127 was a gift from Joseph Sorg (Addgene plasmid # 248630 ; http://n2t.net/addgene:248630 ; RRID:Addgene_248630)

  • For your References section:

    Theophylline-based control of repA on a Clostridioides difficile plasmid for use in allelic exchange. Brehm JN, Sorg JA. Anaerobe. 2024 Apr 29;88:102858. doi: 10.1016/j.anaerobe.2024.102858. 10.1016/j.anaerobe.2024.102858 PubMed 38692475
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