pSTTa
(Plasmid
#248647)
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Purpose(Empty Backbone) E. coli protein expression vector encoding 2x N-terminal StrepII tags flanking an 11 residue flexible linker, thrombin and TEV protease sites, and an optional C-terminal FLAG tag.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248647 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepSTTa (from pGEX)
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Backbone manufacturerGE Healthcare
- Backbone size (bp) 4523
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Vector typeBacterial Expression
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Tags
/ Fusion Proteins
- 2x StrepII, Thrombin site, TEV site (N terminal on insert)
- FLAG (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- 2x StrepII, Thrombin site, TEV site (N terminal on insert)
- FLAG (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSTTa was a gift from Sarah Assmann (Addgene plasmid # 248647 ; http://n2t.net/addgene:248647 ; RRID:Addgene_248647) -
For your References section:
Influence of expression and purification protocols on Galpha biochemical activity: kinetics of plant and mammalian G protein cycles. Gookin TE, Chakravorty D, Assmann SM. Front Mol Biosci. 2025 Apr 7;12:1513660. doi: 10.3389/fmolb.2025.1513660. eCollection 2025. 10.3389/fmolb.2025.1513660 PubMed 40260404
