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pSTTa-GPA1-Q222L
(Plasmid #248650)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248650 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pSTTa (from pGEX)
  • Backbone manufacturer
    GE Healthcare
  • Backbone size w/o insert (bp) 4523
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GPA1
  • Alt name
    AtGPA1
  • Alt name
    AT2G26300
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1152
  • Mutation
    Q222L
  • Entrez Gene
    GP ALPHA 1 (a.k.a. AT2G26300, ARABIDOPSIS THALIANA G PROTEIN ALPHA SUBUNIT 1, ATGPA1, G PROTEIN ALPHA SUBUNIT, G PROTEIN ALPHA SUBUNIT 1, G protein alpha subunit 1, GPA1, T1D16.6, T1D16_6)
  • Promoter tac
  • Tag / Fusion Protein
    • 2x StrepII, Thrombin site, TEV site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BspEI/Kpn2I (not destroyed)
  • 5′ sequencing primer GEXtac (CAAATATTCTGAAATGAGCTGTTGACAA)
  • 3′ sequencing primer pGEX 3'
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Additional details regarding the physiological roles of this mutant GPA1 protein can be found at PMID: 31690635.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSTTa-GPA1-Q222L was a gift from Sarah Assmann (Addgene plasmid # 248650 ; http://n2t.net/addgene:248650 ; RRID:Addgene_248650)

  • For your References section:

    Influence of expression and purification protocols on Galpha biochemical activity: kinetics of plant and mammalian G protein cycles. Gookin TE, Chakravorty D, Assmann SM. Front Mol Biosci. 2025 Apr 7;12:1513660. doi: 10.3389/fmolb.2025.1513660. eCollection 2025. 10.3389/fmolb.2025.1513660 PubMed 40260404
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