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pSTTa-GNAI1
(Plasmid #248651)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248651 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pSTTa (from pGEX)
  • Backbone manufacturer
    GE Healthcare
  • Backbone size w/o insert (bp) 4523
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GNAI1
  • Alt name
    G_i1
  • Alt name
    ENSG00000127955
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1065
  • Entrez Gene
    GNAI1 (a.k.a. Gi, HG1B, NEDHISB)
  • Promoter tac
  • Tag / Fusion Protein
    • 2x StrepII, Thrombin site, TEV site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BspEI/Kpn2I (not destroyed)
  • 5′ sequencing primer GEXtac (CAAATATTCTGAAATGAGCTGTTGACAA)
  • 3′ sequencing primer pGEX 3'
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Genscript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

In PMID: 40260404 codon harmonized and native sequence versions of GNAI1 are described as displaying identical activity, this distributed version carries the native sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSTTa-GNAI1 was a gift from Sarah Assmann (Addgene plasmid # 248651 ; http://n2t.net/addgene:248651 ; RRID:Addgene_248651)

  • For your References section:

    Influence of expression and purification protocols on Galpha biochemical activity: kinetics of plant and mammalian G protein cycles. Gookin TE, Chakravorty D, Assmann SM. Front Mol Biosci. 2025 Apr 7;12:1513660. doi: 10.3389/fmolb.2025.1513660. eCollection 2025. 10.3389/fmolb.2025.1513660 PubMed 40260404
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