pDEST-GBKT7-GNAI1
(Plasmid #248653)

• Purpose: Yeast 2-hybrid vector encoding human heterotrimeric G protein GNAI1 fused to the GAL4 DNA binding domain.
• Depositing Lab: Sarah Assmann
• Publication: Assman lab Y2H vectors (unpublished)

Backbone

• Vector backbone: pGBKT7 (Gateway adapted)
• Backbone manufacturer: Takara
• Backbone size w/o insert (bp): 9016
• Vector type: Yeast Expression ; Yeast 2-hybrid
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GNAI1
• Alt name: G_i1
• Alt name: ENSG00000127955
• Species: H. sapiens (human)
• Insert Size (bp): 1065
• Entrez Gene: GNAI1 (a.k.a. Gi, HG1B, NEDHISB)
• Tag / Fusion Protein:
  • GAL4 binding domain, Myc (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Gal4 N-term
• 3′ sequencing primer: pHybLex Reverse

Resource Information

• Supplemental Documents:
  • pDEST-GBKT7-GNAI1.gb
• A portion of this plasmid was derived from a plasmid made by: Genscript

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry