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pDEST-GBKT7
(Plasmid #248655)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248655 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pGBKT7 (Gateway adapted)
  • Backbone manufacturer
    Takara
  • Backbone size (bp) 9016
  • Vector type
    Yeast Expression ; Yeast 2-hybrid
  • Selectable markers
    TRP1
  • Tag / Fusion Protein
    • GAL4 binding domain, Myc (N terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • GAL4 binding domain, Myc (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid originated from John Doonan's laboratory and is provided, with permission, as an experimental control for pDEST-GBKT7-GNAI1 and pDEST-GBKT7-GNAO1 plasmids that are described as a teaching resource. For all other uses please cite PMID: 17911168.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST-GBKT7 was a gift from Sarah Assmann & John Doonan (Addgene plasmid # 248655 ; http://n2t.net/addgene:248655 ; RRID:Addgene_248655)

  • For your References section:

    Arabidopsis POT1A interacts with TERT-V(I8), an N-terminal splicing variant of telomerase. Rossignol P, Collier S, Bush M, Shaw P, Doonan JH. J Cell Sci. 2007 Oct 15;120(Pt 20):3678-87. doi: 10.1242/jcs.004119. Epub 2007 Oct 2. 10.1242/jcs.004119 PubMed 17911168
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