pDEST-GADT7-RGS19
(Plasmid #248656)

• Purpose: Yeast 2-hybrid vector encoding the human regulator of G protein signaling RGS19 fused to the GAL4 DNA activation domain.
• Depositing Lab: Sarah Assmann
• Publication: Assman lab Y2H vectors (unpublished)

Backbone

• Vector backbone: pGADT7 (Gateway adapted)
• Backbone manufacturer: Takara
• Backbone size w/o insert (bp): 9700
• Vector type: Yeast Expression ; Yeast 2-hybrid
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RGS19
• Alt name: GAIP
• Alt name: ENSG00000171700
• Species: H. sapiens (human)
• Insert Size (bp): 654
• Entrez Gene: RGS19 (a.k.a. GAIP, RGSGAIP)
• Tag / Fusion Protein:
  • GAL4 activation domain, HA (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Gal4-AD
• 3′ sequencing primer: pHybLex Reverse

Resource Information

• Supplemental Documents:
  • pDEST-GADT7-RGS19.gb
• A portion of this plasmid was derived from a plasmid made by: Genscript

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry