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pCDNA5-FRT/TO-NLS-VHL-miniTurbo-3xFLAG
(Plasmid #248670)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248670 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA5/FRT
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VHL
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    639
  • Entrez Gene
    VHL (a.k.a. HRCA1, RCA1, VHL1, pVHL)
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • FLAG-miniTurbo (C terminal on insert)

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

In-fusion cloning

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA5-FRT/TO-NLS-VHL-miniTurbo-3xFLAG was a gift from Cheryl Arrowsmith (Addgene plasmid # 248670 ; http://n2t.net/addgene:248670 ; RRID:Addgene_248670)

  • For your References section:

    Characterization of PROTAC specificity and endogenous protein interactomes using ProtacID. Shrestha S, Maitland MER, Jing L, Duan S, Nie DY, St-Germain J, Kanaris M, Barsyte-Lovejoy D, Arrowsmith CH, Raught B. Nat Commun. 2025 Aug 29;16(1):8089. doi: 10.1038/s41467-025-63357-7. 10.1038/s41467-025-63357-7 PubMed 40883309
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