pACEBac_eIF2_gamma
(Plasmid
#248693)
-
PurposeExpress the human eIF2 gamma subunit in insect cells or clone it in other vectors
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248693 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backbonepACEBac1
-
Backbone manufacturerGeneva Biotech
-
Vector typeInsect Expression
Growth in Bacteria
-
Bacterial Resistance(s)Gentamicin, 10 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameeIF2 gamma
-
Alt nameeIF2S3
-
SpeciesH. sapiens (human)
-
MutationWT
-
Entrez GeneEIF2S3 (a.k.a. EIF2, EIF2G, EIF2gamma, MEHMO, MRXSBRK, eIF-2gA, eIF2gX)
- Promoter Polyhedrin
-
Tag
/ Fusion Protein
- 3C STREP 2 (C terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer ATGGCGGGCGGAGAAGCTG
- (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pACEBac_eIF2_gamma was a gift from Venki Ramakrishnan (Addgene plasmid # 248693 ; http://n2t.net/addgene:248693 ; RRID:Addgene_248693) -
For your References section:
Purification and characterization of recombinant human translation initiation factor eIF3. Diaz-Lopez I, Gordiyenko Y, Zuber PK, Li X, Ramakrishnan V. Protein Sci. 2026 Jan;35(1):e70388. doi: 10.1002/pro.70388. 10.1002/pro.70388 PubMed 41432235