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pSpyB-Tn7sg
(Plasmid #248694)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248694 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pSEVA231
  • Backbone manufacturer
    SEVA
  • Backbone size w/o insert (bp) 3272
  • Total vector size (bp) 3292
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tn7sg
  • gRNA/shRNA sequence
    GTATGCTTTTTCACAGCATA
  • Species
    Synthetic
  • Promoter BG35

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (not destroyed)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer PS-1: AGGGCGGCGGATTTGTCC
  • 3′ sequencing primer PS-2: GCGGCAACCGAGCGTTC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSpyB-Tn7sg was a gift from Larry Halverson (Addgene plasmid # 248694 ; http://n2t.net/addgene:248694 ; RRID:Addgene_248694)

  • For your References section:

    Optimized CRISPR Interference System for Investigating Pseudomonas alloputida Genes Involved in Rhizosphere Microbiome Assembly. Roghair Stroud MN, Vang DX, Halverson LJ. ACS Synth Biol. 2024 Aug 20. doi: 10.1021/acssynbio.4c00312. 10.1021/acssynbio.4c00312 PubMed 39163848
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