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pAAVS1-P-6XPyltRNA-dCas9K1151TAG-IRESPylRS
(Plasmid #248695)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248695 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAAVS1
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dead CRISPR/Cas9, MbPylRS
  • Species
    H. sapiens (human)
  • Promoter CAG

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid contains a Q285K mutation in Cas9. This mutation is not expected to impact plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAVS1-P-6XPyltRNA-dCas9K1151TAG-IRESPylRS was a gift from Shuibing Chen (Addgene plasmid # 248695 ; http://n2t.net/addgene:248695 ; RRID:Addgene_248695)

  • For your References section:

    A chemical epigenetic tool to probe site-specific DNA-binding protein complexes. Zhu J, Li Z, Xue D, Meng Z, Shao S, Pulecio J, Zhang G, Huangfu D, Evans T, Chen S, Schultz PG. Proc Natl Acad Sci U S A. 2025 Oct 7;122(40):e2509021122. doi: 10.1073/pnas.2509021122. Epub 2025 Oct 2. 10.1073/pnas.2509021122 PubMed 41037631
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