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pJMP2656
(Plasmid #248701)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248701 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    R6Kγ
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BW25141
  • Growth instructions
    Plasmid contains both Ampicillin (backbone) and Chloramphenicol (transposon) resistance genes. Either antibiotic can be used for plasmid propagation but Chloramphenicol must be used for selection of downstream transposition events.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    empty sgRNA
  • gRNA/shRNA sequence
    empty sgRNA

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Needs to be grown and distributed in E. coli BW25141 (pir+ strain; required for plasmids with R6K origin). Plasmid was deposited in a version of BW25141 that has an anti-CRISPR protein on its chromosome.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJMP2656 was a gift from Jason Peters (Addgene plasmid # 248701 ; http://n2t.net/addgene:248701 ; RRID:Addgene_248701)

  • For your References section:

    Orthogonal chemical genomics approaches reveal genomic targets for increasing anaerobic chemical tolerance in Zymomonas mobilis. Eckmann JB, Enright Steinberger AL, Davies M, Whelan E, Myers KS, Robinson ML, Banta AB, Lal PB, Coon JJ, Sato TK, Kiley PJ, Peters JM. mSystems. 2025 Dec 4:e0100125. doi: 10.1128/msystems.01001-25. 10.1128/msystems.01001-25 PubMed 41342542
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