pEM526
(Plasmid
#248740)
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PurposeTranscriptional reporter backbone/entry vector with mGL and constitutive mScarlet-I3 in pNBU2 in pNBU2 integrative Bacteroides plasmid
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248740 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepNBU2
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Invitrogen One Shot PIR2 Chemically Competent
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePBfP1E6-RBS1PLP-mScarletI3-rrnBT2-[GG insertion site]
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEM526 was a gift from Carolina Tropini (Addgene plasmid # 248740 ; http://n2t.net/addgene:248740 ; RRID:Addgene_248740)