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pJB161
(Plasmid #248754)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248754 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pNBU2
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Invitrogen One Shot PIR2 Chemically Competent
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-160xTetO-T7Te-rrnBT1-PopuA-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2
  • Species
    Synthetic

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results differ from the full sequence information provided by the depositing laboratory, where Addgene long read sequencing finds x5 TetOs are deleted from the x160 TetO feature. The depositor confirms that this change is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJB161 was a gift from Carolina Tropini (Addgene plasmid # 248754 ; http://n2t.net/addgene:248754 ; RRID:Addgene_248754)
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