pEM352
(Plasmid
#248764)
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Purpose160xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248764 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonep15A
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameT7Te-rrnBT1-160xTetO
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that Addgene's sequencing results differ from the full sequence information provided by the depositing laboratory, where Addgene's long read sequencing finds x5 TetOs are deleted from the x160 TetO feature. The depositor confirms that this change is not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEM352 was a gift from Carolina Tropini (Addgene plasmid # 248764 ; http://n2t.net/addgene:248764 ; RRID:Addgene_248764)