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pcDNA3.1-N-ALFA-bRho-EGFP
(Plasmid #248792)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248792 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 7200
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RHO
  • Species
    B. taurus (bovine)
  • Entrez Gene
    RHO
  • Tags / Fusion Proteins
    • ALFA (N terminal on insert)
    • EGFP (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was generated from the bovine pcDNA3.1-Rho-EGFP-ETSQVAPA plasmid that was obtained from Addgene (#45399).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-N-ALFA-bRho-EGFP was a gift from Michael Robichaux (Addgene plasmid # 248792 ; http://n2t.net/addgene:248792 ; RRID:Addgene_248792)

  • For your References section:

    Super-resolution microscopy reveals a Rab6a-dependent trafficking hub for rhodopsin at the mammalian rod photoreceptor Golgi. Hekmatara M, Thompson SL, Haggerty KN, Hagen S, Brothers BA, Daniels B, Luxardi G, Moshiri A, Deng WT, Robichaux MA. bioRxiv [Preprint]. 2025 Sep 25:2025.09.24.678292. doi: 10.1101/2025.09.24.678292. 10.1101/2025.09.24.678292 PubMed 41040232
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