pLenti CMV GFP Puro_GFP-shortENSA
(Plasmid
#248796)
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PurposeOverexpression of short ENSA
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248796 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepLenti-CMV-GFP-Puro (658-5)
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Backbone manufacturerEric Campeau, Paul Kaufman (Addgene # 17448)
- Backbone size w/o insert (bp) 7860
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameENSA short isoform
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SpeciesH. sapiens (human)
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Insert Size (bp)1129
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Entrez GeneENSA (a.k.a. ARPP-19e)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site Sal1 (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.05.08.652831 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti CMV GFP Puro_GFP-shortENSA was a gift from Maayan Salton (Addgene plasmid # 248796 ; http://n2t.net/addgene:248796 ; RRID:Addgene_248796)