pLenti-CMV-GFP-Puro-Arpp19-short
(Plasmid
#248798)
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PurposeOverexpression of ARPP19 short
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248798 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepLenti-CMV-GFP-Puro (658-5)
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Backbone manufacturerEric Campeau, Paul Kaufman (Addgene # 17448)
- Backbone size w/o insert (bp) 7860
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameARPP19 short isoform
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SpeciesH. sapiens (human)
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Insert Size (bp)353
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Entrez GeneARPP19 (a.k.a. ARPP-16, ARPP-19, ARPP16, ENSAL)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsrGI (unknown if destroyed)
- 3′ cloning site Sal1 (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.05.08.652831 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-CMV-GFP-Puro-Arpp19-short was a gift from Maayan Salton (Addgene plasmid # 248798 ; http://n2t.net/addgene:248798 ; RRID:Addgene_248798)