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His6-mCherry-Flag-LplAW37V and L172A
(Plasmid #248803)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248803 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression
  • Selectable markers
    mTagBFP2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LplA
  • Species
    E. coli
  • Mutation
    W37V and L172A
  • Tag / Fusion Protein
    • 6xHis-mCherry-Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.05.03.652001 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His6-mCherry-Flag-LplAW37V and L172A was a gift from Alice Ting (Addgene plasmid # 248803 ; http://n2t.net/addgene:248803 ; RRID:Addgene_248803)

  • For your References section:

    Computational design of conformation-biasing mutations to alter protein functions. Cavanagh PE, Xue AG, Dai SA, Qiang A, Matsui T, Ting AY. Science. 2026 Jan 8:eadv7953. doi: 10.1126/science.adv7953. 10.1126/science.adv7953 PubMed 41505504
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