P2802
(Plasmid
#248828)
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PurposeAll repressors for the WTC847 system.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248828 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepRG207
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Vector typeYeast Expression
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Selectable markersLYS2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameLexA-L7-hER(L387M), LexA-hER-adh1tail
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Alt namePACT1_LexA-L7-hER(L387M)_tCyc1_P7SulA.1_LexA-hER-adh1tail_tCyc1
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SpeciesS. cerevisiae (budding yeast)
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MutationLexA-L7-hER: L387M
- Promoter pACT1 (LexA-L7-hER(L387M)), P7SulA.1(LexA-hER-adh1tail)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer M13f
- 3′ sequencing primer M13r
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
P2802 was a gift from Joerg Stelling (Addgene plasmid # 248828 ; http://n2t.net/addgene:248828 ; RRID:Addgene_248828)