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P2880
(Plasmid #248830)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248830 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pRG201
  • Vector type
    Yeast Expression
  • Selectable markers
    MET15

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    LacI(6m), LacI(5m)
  • Alt name
    PRnr2_LacI(T167A,W220F,M254L,V119I,Q60G,M242L,NLS)_tFum1_P4Lacn.2_LacI(T167A,W220F,M254L,Q60G,M242L)_tCyc1
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    LacI(6m): T167A, W220F, M254L, V119I, Q60G, M242L; LacI(5m): T167A, W220F, M254L, Q60G, M242L
  • Promoter pRnr2 (LacI(6m), P4Lacn.2 (LacI(5m))
  • Tag / Fusion Protein
    • SV40NLS on LacI(6m) (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    P2880 was a gift from Joerg Stelling (Addgene plasmid # 248830 ; http://n2t.net/addgene:248830 ; RRID:Addgene_248830)
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