P2072
(Plasmid
#248832)
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PurposeLac12 for IPTG import
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248832 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepRG201
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Vector typeYeast Expression
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Selectable markersMET15
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameLac12
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Alt namePACT1_Lac12_tCyc1
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SpeciesS. cerevisiae (budding yeast)
- Promoter pACT1
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13f
- 3′ sequencing primer M13r
- (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
P2072 was a gift from Joerg Stelling (Addgene plasmid # 248832 ; http://n2t.net/addgene:248832 ; RRID:Addgene_248832)