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pAT002
(Plasmid #248842)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248842 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTwist Amp High Copy
  • Backbone manufacturer
    Twist
  • Backbone size w/o insert (bp) 3948
  • Total vector size (bp) 5089
  • Vector type
    zebrafish mRNA expression using SP6

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    2x Lyn Kinase membrane localization sequence
  • Species
    Synthetic
  • Insert Size (bp)
    126
  • Tag / Fusion Protein
    • mNeonGreen (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    mNeonGreen (doi: 10.1038/nmeth.2413) was codon optimized using iCodon (https://bazzinilab.shinyapps.io/icodon/) and synthesized at Twist

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAT002 was a gift from Gokul Upadhyayula (Addgene plasmid # 248842 ; http://n2t.net/addgene:248842 ; RRID:Addgene_248842)

  • For your References section:

    Systematic comparison of mStayGold and mGold2 variants for live imaging in zebrafish. Tschanz A, Matus DQ, Swinburne I. MicroPubl Biol. 2025. 10.17912/micropub.biology.001847. 10.17912/micropub.biology.001847
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