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GST-tag ANKIB1 binding E2 variant L3AN-1
(Plasmid #248852)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248852 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pGEX
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GST-L3AN-1
  • Species
    Synthetic
  • Insert Size (bp)
    465
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-tag ANKIB1 binding E2 variant L3AN-1 was a gift from Brenda Schulman (Addgene plasmid # 248852 ; http://n2t.net/addgene:248852 ; RRID:Addgene_248852)

  • For your References section:

    E2 variants for probing E3 ubiquitin ligase activities. Du J, Andree GA, Horn-Ghetko D, Stier L, Singh J, Kostrhon S, Kiss L, Mann M, Sidhu SS, Schulman BA. Proc Natl Acad Sci U S A. 2026 Jan 6;123(1):e2524899122. doi: 10.1073/pnas.2524899122. Epub 2026 Jan 2. 10.1073/pnas.2524899122 PubMed 41481455
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