pETDuet::MGYFPDa829/MGYFPDa829I
(Plasmid
#248877)
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PurposeExpression of MGYFPDa829 and its immunity protein in bacterial cells for protein purification
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248877 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepETDuet
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name6His-MGYFPDa829, MGYFPDa829I
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pETDuet::MGYFPDa829/MGYFPDa829I was a gift from Brian Liau (Addgene plasmid # 248877 ; http://n2t.net/addgene:248877 ; RRID:Addgene_248877) -
For your References section:
Coupling CRISPR scanning with targeted chromatin accessibility profiling using a double-stranded DNA deaminase. Roh H, Shen SP, Hu Y, Kwok HS, Siegenfeld AP, Lee C, Zepeda MU, Guo CJ, Roseman SA, Comenho C, Sankaran VG, Buenrostro JD, Liau BB. Nat Methods. 2025 Oct;22(10):2083-2093. doi: 10.1038/s41592-025-02811-2. Epub 2025 Sep 11. 10.1038/s41592-025-02811-2 PubMed 40935921