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pJTB72
(Plasmid #248908)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248908 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET-30b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5417
  • Total vector size (bp) 6584
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SMK1 Mitogen-activated Protein Kinase
  • Alt name
    MAPK, Smk1
  • Species
    S. cerevisiae (budding yeast); Sequence is from the SK1 isolate of S. cerevisiae and will differ by 1 non-synonomous/several synonymous changes
  • Insert Size (bp)
    1167
  • GenBank ID
    NP_015379.1
  • Entrez Gene
    SMK1 (a.k.a. YPR054W)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer standard T7 promoter primer
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJTB72 was a gift from Edward Winter (Addgene plasmid # 248908 ; http://n2t.net/addgene:248908 ; RRID:Addgene_248908)

  • For your References section:

    Ssp2 Binding Activates the Smk1 Mitogen-Activated Protein Kinase. Tio CW, Omerza G, Phillips T, Lou HJ, Turk BE, Winter E. Mol Cell Biol. 2017 May 2;37(10):e00607-16. doi: 10.1128/MCB.00607-16. Print 2017 May 15. 10.1128/MCB.00607-16 PubMed 28223369
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