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pZK6
(Plasmid #248911)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248911 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pACYCDuet-1 w/NheI site eliminated
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4008
  • Total vector size (bp) 5959
  • Modifications to backbone
    The NheI site in pACYC-DUET was eliminated from pACYC-DUET which ws then used to clone insert as described below.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Isc10
  • Alt name
    Isc10, Inhibitor of the Smk1 MAPK
  • Species
    S. cerevisiae (budding yeast); Sequence is optimized for bacteria to produce the Isc10 protein from yeast (protein sequence accession below)
  • Insert Size (bp)
    1945
  • GenBank ID
    NP_011107.1
  • Entrez Gene
    ISC10 (a.k.a. YER180C)
  • Promoter T7
  • Tag / Fusion Protein
    • maltose binding protein (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer standard T7 promoter primer
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZK6 was a gift from Edward Winter (Addgene plasmid # 248911 ; http://n2t.net/addgene:248911 ; RRID:Addgene_248911)

  • For your References section:

    Isc10, an Inhibitor That Links the Anaphase-Promoting Complex to a Meiosis-Specific Mitogen-Activated Protein Kinase. Rimal A, Kamdar ZP, Tio CW, Winter E. Mol Cell Biol. 2020 Jul 29;40(16). pii: MCB.00097-20. doi: 10.1128/MCB.00097-20. Print 2020 Jul 29. 10.1128/MCB.00097-20 PubMed 32423992
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