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pJTB122
(Plasmid #248913)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248913 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pACYC-DUET
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4008
  • Total vector size (bp) 4995
  • Modifications to backbone
    none
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CAK1
  • Alt name
    Cdk activating kinase, Cak1
  • Species
    S. cerevisiae (budding yeast)
  • GenBank ID
    NM_001179937.1
  • Entrez Gene
    CAK1 (a.k.a. YFL029C, CIV1)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer standard T7 promoter primer
  • 3′ sequencing primer standard T7 promoter primer
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJTB122 was a gift from Edward Winter (Addgene plasmid # 248913 ; http://n2t.net/addgene:248913 ; RRID:Addgene_248913)

  • For your References section:

    Isc10, an inhibitor of the Smk1 MAPK, prevents activation loop autophosphorylation and substrate phosphorylation through separate mechanisms. Rimal A, Swayne TM, Kamdar ZP, Tewey MA, Winter E. J Biol Chem. 2022 Oct;298(10):102450. doi: 10.1016/j.jbc.2022.102450. Epub 2022 Sep 3. 10.1016/j.jbc.2022.102450 PubMed 36063999
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