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GST-p25
(Plasmid #24896)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24896 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p25
  • Alt name
    p35 Truncated
  • Species
    H. sapiens (human)
  • Mutation
    N-terminal truncated version of p35, contains aa 99-307
  • Entrez Gene
    CDK5R1 (a.k.a. CDK5P35, CDK5R, NCK5A, p23, p25, p35, p35nck5a)
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13-Puc_Rev, pGEX5'
  • 3′ sequencing primer pGEX3'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Li-Huei Tsai
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-p25 was a gift from Harish Pant (Addgene plasmid # 24896 ; http://n2t.net/addgene:24896 ; RRID:Addgene_24896)
  • For your References section:

    Cyclin-dependent kinase 5 (cdk5) activation requires interaction with three domains of p35. Amin ND, Albers W, Pant HC. J Neurosci Res. 2002 Feb 1. 67(3):354-62. 10.1002/jnr.10116 PubMed 11813240