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pDBB63
(Plasmid #248966)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248966 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    PBgg-Hygro
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NShh-CD4-IRES2-H2B-mScarletI
  • Species
    M. musculus (mouse)
  • Promoter UASybTATA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.06.11.598403 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDBB63 was a gift from James Briscoe (Addgene plasmid # 248966 ; http://n2t.net/addgene:248966 ; RRID:Addgene_248966)

  • For your References section:

    Investigating morphogen and patterning dynamics with optogenetic control of morphogen production. Benzinger D, Briscoe J. Dev Cell. 2025 Aug 19:S1534-5807(25)00495-2. doi: 10.1016/j.devcel.2025.07.019. 10.1016/j.devcel.2025.07.019 PubMed 40848721
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