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p17-GFP-CYRI-B
(Plasmid #248969)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248969 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    Expression Vector
  • Backbone size w/o insert (bp) 4800
  • Total vector size (bp) 6360
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cyri-B
  • Alt name
    Fam49B
  • Species
    H. sapiens (human)
  • Entrez Gene
    CYRIB (a.k.a. BM-009, CYRI, CYRI-B, FAM49B, L1)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    This was cloned in Laura Machesky's lab by Loic Fort, at the CRUK Scotland Institute

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Laura Machesky's Lab is now in the Department of Biochemistry at the University of Cambridge

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p17-GFP-CYRI-B was a gift from Laura Machesky (Addgene plasmid # 248969 ; http://n2t.net/addgene:248969 ; RRID:Addgene_248969)

  • For your References section:

    CYRI-A limits invasive migration through macropinosome formation and integrin uptake regulation. Le AH, Yelland T, Paul NR, Fort L, Nikolaou S, Ismail S, Machesky LM. J Cell Biol. 2021 Sep 6;220(9). pii: 212437. doi: 10.1083/jcb.202012114. Epub 2021 Jun 24. 10.1083/jcb.202012114 PubMed 34165494
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