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MultiCAST-Helper
(Plasmid #248973)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248973 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    R6K origin
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Copy number
    Low Copy

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The CAST Tn6677 CRISPR and transposition genes were derived from pSL1765 (Plasmid #160734).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.10.31.685825 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MultiCAST-Helper was a gift from Matthew Waldor (Addgene plasmid # 248973 ; http://n2t.net/addgene:248973 ; RRID:Addgene_248973)

  • For your References section:

    Adapting CRISPR-associated transposons for rapid and high-throughput reverse genetics. Basta DW, Zingl FG, Yang Y, Nguyen KT, Liu Y-Y, Waldor MK. bioRxiv 2025.10.31.685825 10.1101/2025.10.31.685825
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