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pMSCV-puro-myc-S1-CC1-NP2
(Plasmid #248976)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248976 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pMSCV-puro
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Nucleoplasmic region of SUN2 and coiled coil region and SUN domain of SUN1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Sun1 (a.k.a. 4632417G13Rik, 5730434D03Rik, Unc84a, mKIAA0810)
  • Entrez Gene
    Sun2 (a.k.a. B230369L08Rik, C030011B15, Unc84b)
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.01.15.633301 for the bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-puro-myc-S1-CC1-NP2 was a gift from Wakam Chang (Addgene plasmid # 248976 ; http://n2t.net/addgene:248976 ; RRID:Addgene_248976)
  • For your References section:

    Elevated SUN1 promotes migratory cell polarity defects through mechanically coupling microtubules to the nuclear lamina. Li Y, Zhang Y, Chen M, Antoku S, Ding J, Huang K, Gundersen GG, Chang W. Commun Biol. 2025 Dec 9;9(1):9. doi: 10.1038/s42003-025-09229-8. 10.1038/s42003-025-09229-8 PubMed 41366027