pMSCV-puro-myc-S2-CC2-NP1
(Plasmid
#248978)
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PurposeRetroviral expression vector for myc-tagged mouse SUN1-SUN2 chimera protein (Nucleoplasmic region of SUN1 and coiled coil region and SUN domain of SUN2)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 248978 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepMSCV-puro
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Vector typeRetroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNucleoplasmic region of SUN1 and coiled coil region and SUN domain of SUN2
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SpeciesM. musculus (mouse)
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Entrez GeneSun1 (a.k.a. 4632417G13Rik, 5730434D03Rik, Unc84a, mKIAA0810)
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Entrez GeneSun2 (a.k.a. B230369L08Rik, C030011B15, Unc84b)
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Tag
/ Fusion Protein
- Myc (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.01.15.633301 for the bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCV-puro-myc-S2-CC2-NP1 was a gift from Wakam Chang (Addgene plasmid # 248978 ; http://n2t.net/addgene:248978 ; RRID:Addgene_248978) -
For your References section:
Elevated SUN1 promotes migratory cell polarity defects through mechanically coupling microtubules to the nuclear lamina. Li Y, Zhang Y, Chen M, Antoku S, Ding J, Huang K, Gundersen GG, Chang W. Commun Biol. 2025 Dec 9;9(1):9. doi: 10.1038/s42003-025-09229-8. 10.1038/s42003-025-09229-8 PubMed 41366027