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pKlab1154-HBEGF-2a-3xFLAG-BRCA1(full-length)-D1692H-2a-mRuby3-HBBIVS2-WPRE
(Plasmid #248994)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248994 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    Xential template for knock-in at the HBEGF locus
  • Vector type
    CRISPR
  • Selectable markers
    HBEGF(Exon4mut&Exon5), mRuby9

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BRCA1
  • Species
    H. sapiens (human)
  • Mutation
    D1692H
  • Entrez Gene
    BRCA1 (a.k.a. BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
  • Tag / Fusion Protein
    • 3x-FLAG-V5 (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKlab1154-HBEGF-2a-3xFLAG-BRCA1(full-length)-D1692H-2a-mRuby3-HBBIVS2-WPRE was a gift from Georgios Karras (Addgene plasmid # 248994 ; http://n2t.net/addgene:248994 ; RRID:Addgene_248994)

  • For your References section:

    HSP90 buffers deleterious genetic variations in BRCA1. Gracia B, Zhang XH, Montes P, Pham TC, Huang M, Chen J, Karras GI. Mol Cell. 2025 Nov 19:S1097-2765(25)00867-6. doi: 10.1016/j.molcel.2025.10.026. 10.1016/j.molcel.2025.10.026 PubMed 41265458
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