pKlab1157-HBEGF-2a-3xFLAG-BRCA1(full-length)-P1749S-2a-mRuby3-HBBIVS2-WPRE
(Plasmid #248997)

• Purpose: This plasmid expresses the BRCA1 sequence with P1749S mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
• Depositing Lab: Georgios Karras
• Publication: Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)00867-6. doi: 10.1016/j.molcel.2025.10.026.

Backbone

• Vector backbone: Xential template for knock-in at the HBEGF locus
• Vector type: CRISPR
• Selectable markers: HBEGF(Exon4mut&Exon5), mRuby12

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BRCA1
• Species: H. sapiens (human)
• Mutation: P1749S
• Entrez Gene: BRCA1 (a.k.a. BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
• Tag / Fusion Protein:
  • 3x-FLAG-V5 (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• A portion of this plasmid was derived from a plasmid made by: based on vectors from Li et al. Nat Comms 2021 (PMID: 33479216)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry