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pKlab965-PX459-BRCA1-Nterm-guide1
(Plasmid #249005)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249005 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pVAX1
  • Vector type
    CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • gRNA/shRNA sequence
    TTCATTGGAACAGAAAGAAA
  • Species
    H. sapiens (human)
  • Promoter chicken beta-actin promoter
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Second insert: gRNA followed by gRNA scaffold

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKlab965-PX459-BRCA1-Nterm-guide1 was a gift from Georgios Karras (Addgene plasmid # 249005 ; http://n2t.net/addgene:249005 ; RRID:Addgene_249005)

  • For your References section:

    HSP90 buffers deleterious genetic variations in BRCA1. Gracia B, Zhang XH, Montes P, Pham TC, Huang M, Chen J, Karras GI. Mol Cell. 2025 Nov 19:S1097-2765(25)00867-6. doi: 10.1016/j.molcel.2025.10.026. 10.1016/j.molcel.2025.10.026 PubMed 41265458
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