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pKlab264-pcDNA3-BRCA1(1314-end)-M1783T-V5-3xFLAG
(Plasmid #249017)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249017 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BRCA1(1314aa-end)
  • Species
    H. sapiens (human)
  • Mutation
    M1783T
  • Entrez Gene
    BRCA1 (a.k.a. BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
  • Promoter CMV Promoter
  • Tag / Fusion Protein
    • 3x-FLAG-V5 (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKlab264-pcDNA3-BRCA1(1314-end)-M1783T-V5-3xFLAG was a gift from Georgios Karras (Addgene plasmid # 249017 ; http://n2t.net/addgene:249017 ; RRID:Addgene_249017)

  • For your References section:

    Protein-folding chaperones predict structure-function relationships and cancer risk in BRCA1 mutation carriers. Gracia B, Montes P, Gutierrez AM, Arun B, Karras GI. Cell Rep. 2024 Feb 27;43(2):113803. doi: 10.1016/j.celrep.2024.113803. Epub 2024 Feb 17. 10.1016/j.celrep.2024.113803 PubMed 38368609
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