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AG0057 LGA (DIO-mGlp1r)
(Plasmid #249035)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249035 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    Roth Addgene #50457
  • Backbone manufacturer
    Roth Lab
  • Backbone size w/o insert (bp) 4813
  • Total vector size (bp) 6313
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mGlp1r
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1500
  • Promoter hSyn
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer GCCCAGCCGGACCGCACCA
  • 3′ sequencing primer WPRE-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Twist Biosciences

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AG0057 LGA (DIO-mGlp1r) was a gift from Ali Guler (Addgene plasmid # 249035 ; http://n2t.net/addgene:249035 ; RRID:Addgene_249035)

  • For your References section:

    A Brain Reward Circuit Inhibited By Next-Generation Weight Loss Drugs. Godschall EN, Gungul TB, Sajonia IR, Buyukaksakal AK, Li OD, Ogilvie S, Keeler AB, Tian G, Koita O, Shi Y, Deutsch TCJ, Crook M, Zhang Y, Conley NJ, Webster AN, Calhan OY, Liu W, Akkoub A, Malik K, West KI, Michel-Le S, Karthikeyan A, van Gerven G, Beier KT, Zweifel LS, Patel MK, Campbell JN, Deppmann CD, Guler AD. bioRxiv [Preprint]. 2025 Jun 14:2024.12.12.628169. doi: 10.1101/2024.12.12.628169. 10.1101/2024.12.12.628169 PubMed 39763823
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