pLenti-hETFDH-WT
(Plasmid
#249046)
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PurposeExpression of human wildtype ETFDH
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 249046 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepUltra
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Vector typeLentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameETFDH
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Alt nameETFQO
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SpeciesH. sapiens (human)
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Insert Size (bp)1853
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Entrez GeneETFDH (a.k.a. ETFQO, MADD)
Cloning Information
- Cloning method Other
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byVectorBuilder
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-hETFDH-WT was a gift from Ivan Topisirovic (Addgene plasmid # 249046 ; http://n2t.net/addgene:249046 ; RRID:Addgene_249046) -
For your References section:
Mitochondrial ETF insufficiency drives neoplastic growth by selectively optimizing cancer bioenergetics. Papadopoli D, Palia R, Jovanovic P, Tabariès S, Ciccolini E, Sabourin V, Igelmann S, McLaughlan S, Zhan L, Kim H, Chekkal N, Szkop KJ, Bertomeu T, Zeng J, Vassalakis J, Afzali F, Mzoughi S, Guccione E, Tyers M, Avizonis D, Larsson O, Postovit L-M, Djuranovic S, Ursini-Siegel J, Siegel PM, Pollak M, Topisirovic I. eLife, 2025 [Reviewed Preprint] 14:RP106587 10.7554/eLife.106587.1