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p-mCherry-enCas9(D10A)
(Plasmid #249063)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249063 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    n/a
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    enCas9
  • Species
    Synthetic

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-mCherry-enCas9(D10A) was a gift from John Dueber & David Schaffer (Addgene plasmid # 249063 ; http://n2t.net/addgene:249063 ; RRID:Addgene_249063)

  • For your References section:

    Nickase fidelity drives EvolvR-mediated diversification in mammalian cells. Hurtado JE, Schieferecke AJ, Halperin SO, Guan J, Aidlen D, Schaffer DV, Dueber JE. Nat Commun. 2025 Apr 19;16(1):3723. doi: 10.1038/s41467-025-58414-0. 10.1038/s41467-025-58414-0 PubMed 40253348
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