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pscAAV-KIKO-U6-gTgfbr2-p2a-mCherry-STOPpA-400
(Plasmid #249131)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249131 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pscAAV-CAG-GFP
  • Backbone size w/o insert (bp) 2955
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    gTgfbr2, mCherry
  • gRNA/shRNA sequence
    GTCCACAGGACGATATGCAG
  • Species
    M. musculus (mouse), Synthetic
  • Insert Size (bp)
    2061
  • Entrez Gene
    Tgfbr2 (a.k.a. 1110020H15Rik, DNIIR, RIIDN, TBR-II, TbetaR-II, TbetaRII)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

gTgfbr2 cuts in Exon 1 of Tgfbr2; contains left (400 bp) and right (380 bp) homology arms relative to cut site to facilitate in-frame knock-in of p2a-mCherry

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pscAAV-KIKO-U6-gTgfbr2-p2a-mCherry-STOPpA-400 was a gift from Matthew Krummel (Addgene plasmid # 249131 ; http://n2t.net/addgene:249131 ; RRID:Addgene_249131)
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