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pSA074 Cas9-BFP/HBB_IVS2/BFP
(Plasmid #249135)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249135 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pPD008
  • Vector type
    Mammalian Expression, CRISPR ; Cp36 donor DNA
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9-TagBFP/HBB_IVS2/TagBFP
  • Species
    H. sapiens (human); S. pyogenes Cas9, synthetic
  • Mutation
    HBB IVS2 intron
  • Entrez Gene
    HBB (a.k.a. CD113t-C, ECYT6, beta-globin)
  • Promoter EF1a

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSA074 Cas9-BFP/HBB_IVS2/BFP was a gift from Michael Bassik (Addgene plasmid # 249135 ; http://n2t.net/addgene:249135 ; RRID:Addgene_249135)

  • For your References section:

    Reduced Cas9 transgene silencing by incorporation of intron sequences. Arana S, Du PP, Vaughan-Jackson A, Enright N, Spees K, Valbuena R, Garcia CA, Nguyen T, Venida A, Seczynska M, Bintu L, Lehner PJ, Prolo LM, Bassik MC. Nat Commun. 2025 Nov 27;16(1):10656. doi: 10.1038/s41467-025-65669-0. 10.1038/s41467-025-65669-0 PubMed 41309578
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