pEGFP-N1/Integrin-b5-FL-SGx3-Halo9
(Plasmid #249166)

• Purpose: Mammalian expression vector of human ITGB5 fused with Halo9-tag
• Depositing Lab: Pakorn Kanchanawong
• Publication: Nishimura et al J Cell Sci. 2025 Nov 24:jcs.264110. doi: 10.1242/jcs.264110.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 6507
• Total vector size (bp): 7306
• Modifications to backbone: Substituted EGFP with Halo9
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Integrin beta-5
• Species: H. sapiens (human)
• Insert Size (bp): 2397
• Entrez Gene: ITGB5
• Promoter: CMV
• Tag / Fusion Protein:
  • Halo9 (C terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • pegfp-n1integrin-b5-fl-sgx3-halo9.gb
• A portion of this plasmid was derived from a plasmid made by: Integrin beta5-2xEGFP deposited by Dr. Staffan Strömblad; Addgene plasmid #139779

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There was a truncation (22-amino acid deletion) at the C-terminus in the original plasmid. To obtain an intact full-length ITGB5 gene fragment, we synthesized the missing portion and fused it to the C-terminus via seamless cloning.

Please visit https://doi.org/10.1101/2025.04.17.649301 for bioRxiv preprint.