mApple-Talin1(1–1973)-pdDronpa1.2
(Plasmid #249167)

• Purpose: Mammalian expression vector of talin1 N-terminal fragment fused with pdDronpa1.2
• Depositing Lab: Pakorn Kanchanawong
• Publication: Nishimura et al J Cell Sci. 2025 Nov 24:jcs.264110. doi: 10.1242/jcs.264110.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4654
• Total vector size (bp): 11260
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Talin1
• Species: H. sapiens (human)
• Insert Size (bp): 6606
• Mutation: Deleted amino acids 1974–2541
• Entrez Gene: TLN1 (a.k.a. ILWEQ, TLN, talin-1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • mApple (N terminal on backbone)
  • pdDronpa1.2 (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • t50_talinn10-pddronpa1-2.gb
• A portion of this plasmid was derived from a plasmid made by: Talin1(1–2541)-mEmerald from Dr. Michael Davidson, Addgene #62763

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2025.04.17.649301 for bioRxiv preprint.