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mIFP-Talin1(1–2493)-pdDronpa1.2
(Plasmid #249170)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249170 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4984
  • Total vector size (bp) 13144
  • Modifications to backbone
    EGFP was replaced with mIFP
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Talin1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    7482
  • Mutation
    Deleted amino acids 2494–2541
  • Entrez Gene
    TLN1 (a.k.a. ILWEQ, TLN, talin-1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • mIFP (N terminal on backbone)
    • pdDronpa1.2 (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Talin1(1–2541)-mEmerald from Dr. Michael Davidson, Addgene #62763

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.04.17.649301 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mIFP-Talin1(1–2493)-pdDronpa1.2 was a gift from Pakorn Kanchanawong (Addgene plasmid # 249170 ; http://n2t.net/addgene:249170 ; RRID:Addgene_249170)

  • For your References section:

    Optogenetic control of mechanotransduction based on light-induced homodimerization of talin. Nishimura R, Barnett SFH, Jain K, Huang Z, Goult BT, Kanchanawong P. J Cell Sci. 2025 Nov 24:jcs.264110. doi: 10.1242/jcs.264110. 10.1242/jcs.264110 PubMed 41277405
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